Abstract: Objective This study aims to explore a method for eliminating nonspecific agglutination factors (NSAs) in rhesus monkey serum. Method Firstly, 1% chicken red blood cells and 1% guinea pig red blood cells were used to screen NSAs in rhesus monkey serum, respectively. Then, serum samples showing nonspecific agglutination were mixed with different concentrations ( 1%, 5%, 10%, 15% and 20%) of chicken red blood cells in equal volumes, and the agglutination test was carried out by chicken red blood cells after adsorption treatment at room temperature for 0. 5 h, 1 h and 2-8 ℃ for 2, 4, 6, 8, 12 h, respectively. Ultimately, the conditions of adsorpting and eliminating NSAs in rhesus monkey serum were preliminarily determined and verified, included the best chicken red blood cell concentration, adsorption time, adsorption temperature and elimination stability. Result (1) NSAs were existed in rhesus monkey serum, with a 100% agglutination rate was observed when screened using 1% chicken red blood cells or 1% guinea pig red blood cells. ( 2 ) The rhesus monkey nonspecific agglutination serum was adsorpted with different concentrations of chicken erythrocytes. The elimination rate of NSAs range from 0% -50%, 0% after treated at room temperature for 0. 5 h and1 h, respectively. The elimination rates of NSAs range from 20% -54%, 40% -100%, 90% -100%, 80% -100%, 90% - 100% after treated at 2-8 ℃ for 2 , 4 , 6 , 8 and 12 h, respectively. (3) The elimination rate of NSAs in rhesus monkey serum could reach at 90. 00%and 83. 33%when treated with 5% chicken erythrocytes at 2-8 ℃ for 4 h and 6 h, respectively. Conclusion This study successfully established an efficient, stable and reliable method for eliminating NSAs in rhesus monkey serum using chicken red blood cells adsorption treatment.

Key words: nonspecific agglutination factors, eliminating, hemadsorption, rhesus monkey

摘要： 目的 探索恒河猴血清非特异性凝集因子(NSAs) 的消除方法。 方法 首先分别使用 1% 鸡红细胞和 1% 豚 鼠红细胞对恒河猴血清进行非特异性凝集筛选,然后将非特异性凝集血清样本与不同浓度( 1%、5%、10%、15% 和 20%)的鸡红细胞等体积混匀,分别置室温吸附处理 0. 5、1 h 和 2 ~ 8 ℃ 吸附处理 2、4、6、8 和 12 h 后检测被检血清 凝集情况,初步确定恒河猴血清 NSAs 鸡红细胞吸附消除的条件,并对其进行效果验证,从而最终明确恒河猴血清 NSAs 鸡红细胞吸附消除的最佳红细胞浓度、吸附时间、吸附温度以及消除稳定性。 结果 ( 1) 恒河猴血清中存在 NSAs,使用 1%鸡和 1%豚鼠红细胞对恒河猴血清进行非特异性凝集筛选其凝集率均为 100%。 ( 2) 恒河猴非特异 性凝集血清使用不同浓度鸡红细胞在室温下吸附处理 0. 5 h 后 NSAs 去除率为 0% ~ 50%,处理 1 h 后 NSAs 去除率 均为 0%;在 2 ~ 8 ℃ 下吸附处理 2、4、6、8 和 12 h 后 NSAs 去除率分别为 20% ~ 54%、40% ~ 100%、90% ~ 100%、80% ~ 100%、90% ~ 100%。 (3)60 份恒河猴非特异性凝集血清在 2 ~ 8 ℃ 下使用 5% 鸡红细胞吸附处理 4 和 6 h,NSAs 的 去除率可达 90. 00%和 83. 33%。 结论 本研究成功建立了一种高效、稳定、可靠的恒河猴血清 NSAs 鸡红细胞吸附消除方法。

关键词: 非特异性凝集因子, 消除, 红细胞吸附, 恒河猴